PhytophthoraV1, Main, Exploration, bibRecord, 000709

Over-expression of the Pseudomonas syringae harpin-encoding gene hrpZm confers enhanced tolerance to Phytophthora root and stem rot in transgenic soybean.

Identifieur interne : 000709 ( Main/Exploration ); précédent : 000708; suivant : 000710

Over-expression of the Pseudomonas syringae harpin-encoding gene hrpZm confers enhanced tolerance to Phytophthora root and stem rot in transgenic soybean.

Source :

Transgenic research [ 1573-9368 ] ; 2018.

RBID : pubmed:29728957

Descripteurs français

English descriptors

KwdEn :
- Bacterial Outer Membrane Proteins (genetics), Disease Resistance (genetics), Gene Expression Regulation, Plant (MeSH), Phytophthora (genetics), Phytophthora (pathogenicity), Plant Diseases (genetics), Plant Diseases (parasitology), Plant Stems (genetics), Plant Stems (growth & development), Plant Stems (parasitology), Plants, Genetically Modified (genetics), Plants, Genetically Modified (growth & development), Plants, Genetically Modified (parasitology), Pseudomonas syringae (genetics), Signal Transduction (genetics), Soybeans (genetics), Soybeans (growth & development), Soybeans (parasitology).
MESH :
- chemical , genetics : Bacterial Outer Membrane Proteins.
- genetics : Disease Resistance, Phytophthora, Plant Diseases, Plant Stems, Plants, Genetically Modified, Pseudomonas syringae, Signal Transduction, Soybeans.
- growth & development : Plant Stems, Plants, Genetically Modified, Soybeans.
- parasitology : Plant Diseases, Plant Stems, Plants, Genetically Modified, Soybeans.
- pathogenicity : Phytophthora.
- Gene Expression Regulation, Plant.

Abstract

Phytophthora root and stem rot (PRR) caused by Phytophthora sojae is one of the most devastating diseases reducing soybean (Glycine max) production all over the world. Harpin proteins in many plant pathogenic bacteria were confirmed to enhance disease and insect resistance in crop plants. Here, a harpin protein-encoding gene hrpZpsta from the P. syringae pv. tabaci strain Psta218 was codon-optimized (renamed hrpZm) and introduced into soybean cultivars Williams 82 and Shennong 9 by Agrobacterium-mediated transformation. Three independent transgenic lines over-expressing hrpZm were obtained and exhibited stable and enhanced tolerance to P. sojae infection in T₂-T₄ generations compared to the non-transformed (NT) and empty vector (EV)-transformed plants. Quantitative real-time PCR (qRT-PCR) analysis revealed that the expression of salicylic acid-dependent genes PR1, PR12, and PAL, jasmonic acid-dependent gene PPO, and hypersensitive response (HR)-related genes GmNPR1 and RAR was significantly up-regulated after P. sojae inoculation. Moreover, the activities of defense-related enzymes such as phenylalanine ammonia lyase (PAL), polyphenoloxidase (PPO), peroxidase, and superoxide dismutase also increased significantly in the transgenic lines compared to the NT and EV-transformed plants after inoculation. Our results suggest that over-expression of the hrpZm gene significantly enhances PRR tolerance in soybean by eliciting resistance responses mediated by multiple defense signaling pathways, thus providing an alternative approach for development of soybean varieties with improved tolerance against the soil-borne pathogen PRR.

DOI: 10.1007/s11248-018-0071-4
PubMed: 29728957

Affiliations:

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Le document en format XML

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<author><name sortKey="Jiang, Zhaoyuan" sort="Jiang, Zhaoyuan" uniqKey="Jiang Z" first="Zhaoyuan" last="Jiang">Zhaoyuan Jiang</name>
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<author><name sortKey="Dong, Yingshan" sort="Dong, Yingshan" uniqKey="Dong Y" first="Yingshan" last="Dong">Yingshan Dong</name>
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<author><name sortKey="Pan, Hongyu" sort="Pan, Hongyu" uniqKey="Pan H" first="Hongyu" last="Pan">Hongyu Pan</name>
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<term>Gene Expression Regulation, Plant (MeSH)</term>
<term>Phytophthora (genetics)</term>
<term>Phytophthora (pathogenicity)</term>
<term>Plant Diseases (genetics)</term>
<term>Plant Diseases (parasitology)</term>
<term>Plant Stems (genetics)</term>
<term>Plant Stems (growth & development)</term>
<term>Plant Stems (parasitology)</term>
<term>Plants, Genetically Modified (genetics)</term>
<term>Plants, Genetically Modified (growth & development)</term>
<term>Plants, Genetically Modified (parasitology)</term>
<term>Pseudomonas syringae (genetics)</term>
<term>Signal Transduction (genetics)</term>
<term>Soybeans (genetics)</term>
<term>Soybeans (growth & development)</term>
<term>Soybeans (parasitology)</term>
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<keywords scheme="KwdFr" xml:lang="fr"><term>Maladies des plantes (génétique)</term>
<term>Maladies des plantes (parasitologie)</term>
<term>Phytophthora (génétique)</term>
<term>Phytophthora (pathogénicité)</term>
<term>Protéines de la membrane externe bactérienne (génétique)</term>
<term>Pseudomonas syringae (génétique)</term>
<term>Régulation de l'expression des gènes végétaux (MeSH)</term>
<term>Résistance à la maladie (génétique)</term>
<term>Soja (croissance et développement)</term>
<term>Soja (génétique)</term>
<term>Soja (parasitologie)</term>
<term>Tiges de plante (croissance et développement)</term>
<term>Tiges de plante (génétique)</term>
<term>Tiges de plante (parasitologie)</term>
<term>Transduction du signal (génétique)</term>
<term>Végétaux génétiquement modifiés (croissance et développement)</term>
<term>Végétaux génétiquement modifiés (génétique)</term>
<term>Végétaux génétiquement modifiés (parasitologie)</term>
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</keywords>
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<term>Tiges de plante</term>
<term>Végétaux génétiquement modifiés</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Disease Resistance</term>
<term>Phytophthora</term>
<term>Plant Diseases</term>
<term>Plant Stems</term>
<term>Plants, Genetically Modified</term>
<term>Pseudomonas syringae</term>
<term>Signal Transduction</term>
<term>Soybeans</term>
</keywords>
<keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Plant Stems</term>
<term>Plants, Genetically Modified</term>
<term>Soybeans</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Maladies des plantes</term>
<term>Phytophthora</term>
<term>Protéines de la membrane externe bactérienne</term>
<term>Pseudomonas syringae</term>
<term>Résistance à la maladie</term>
<term>Soja</term>
<term>Tiges de plante</term>
<term>Transduction du signal</term>
<term>Végétaux génétiquement modifiés</term>
</keywords>
<keywords scheme="MESH" qualifier="parasitologie" xml:lang="fr"><term>Maladies des plantes</term>
<term>Soja</term>
<term>Tiges de plante</term>
<term>Végétaux génétiquement modifiés</term>
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<term>Soybeans</term>
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</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Gene Expression Regulation, Plant</term>
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<front><div type="abstract" xml:lang="en">Phytophthora root and stem rot (PRR) caused by Phytophthora sojae is one of the most devastating diseases reducing soybean (Glycine max) production all over the world. Harpin proteins in many plant pathogenic bacteria were confirmed to enhance disease and insect resistance in crop plants. Here, a harpin protein-encoding gene hrpZpsta from the P. syringae pv. tabaci strain Psta218 was codon-optimized (renamed hrpZm) and introduced into soybean cultivars Williams 82 and Shennong 9 by Agrobacterium-mediated transformation. Three independent transgenic lines over-expressing hrpZm were obtained and exhibited stable and enhanced tolerance to P. sojae infection in T<sub>2</sub>
-T<sub>4</sub>
 generations compared to the non-transformed (NT) and empty vector (EV)-transformed plants. Quantitative real-time PCR (qRT-PCR) analysis revealed that the expression of salicylic acid-dependent genes PR1, PR12, and PAL, jasmonic acid-dependent gene PPO, and hypersensitive response (HR)-related genes GmNPR1 and RAR was significantly up-regulated after P. sojae inoculation. Moreover, the activities of defense-related enzymes such as phenylalanine ammonia lyase (PAL), polyphenoloxidase (PPO), peroxidase, and superoxide dismutase also increased significantly in the transgenic lines compared to the NT and EV-transformed plants after inoculation. Our results suggest that over-expression of the hrpZm gene significantly enhances PRR tolerance in soybean by eliciting resistance responses mediated by multiple defense signaling pathways, thus providing an alternative approach for development of soybean varieties with improved tolerance against the soil-borne pathogen PRR.</div>
</front>
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<Title>Transgenic research</Title>
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<ArticleTitle>Over-expression of the Pseudomonas syringae harpin-encoding gene hrpZm confers enhanced tolerance to Phytophthora root and stem rot in transgenic soybean.</ArticleTitle>
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</Pagination>
<ELocationID EIdType="doi" ValidYN="Y">10.1007/s11248-018-0071-4</ELocationID>
<Abstract><AbstractText>Phytophthora root and stem rot (PRR) caused by Phytophthora sojae is one of the most devastating diseases reducing soybean (Glycine max) production all over the world. Harpin proteins in many plant pathogenic bacteria were confirmed to enhance disease and insect resistance in crop plants. Here, a harpin protein-encoding gene hrpZpsta from the P. syringae pv. tabaci strain Psta218 was codon-optimized (renamed hrpZm) and introduced into soybean cultivars Williams 82 and Shennong 9 by Agrobacterium-mediated transformation. Three independent transgenic lines over-expressing hrpZm were obtained and exhibited stable and enhanced tolerance to P. sojae infection in T<sub>2</sub>
-T<sub>4</sub>
 generations compared to the non-transformed (NT) and empty vector (EV)-transformed plants. Quantitative real-time PCR (qRT-PCR) analysis revealed that the expression of salicylic acid-dependent genes PR1, PR12, and PAL, jasmonic acid-dependent gene PPO, and hypersensitive response (HR)-related genes GmNPR1 and RAR was significantly up-regulated after P. sojae inoculation. Moreover, the activities of defense-related enzymes such as phenylalanine ammonia lyase (PAL), polyphenoloxidase (PPO), peroxidase, and superoxide dismutase also increased significantly in the transgenic lines compared to the NT and EV-transformed plants after inoculation. Our results suggest that over-expression of the hrpZm gene significantly enhances PRR tolerance in soybean by eliciting resistance responses mediated by multiple defense signaling pathways, thus providing an alternative approach for development of soybean varieties with improved tolerance against the soil-borne pathogen PRR.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Du</LastName>
<ForeName>Qian</ForeName>
<Initials>Q</Initials>
<AffiliationInfo><Affiliation>College of Plant Science, Jilin University, Changchun, 130062, China.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Jilin Academy of Agricultural Sciences, Changchun, 130033, China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Yang</LastName>
<ForeName>Xiangdong</ForeName>
<Initials>X</Initials>
<AffiliationInfo><Affiliation>Jilin Academy of Agricultural Sciences, Changchun, 130033, China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Zhang</LastName>
<ForeName>Jinhua</ForeName>
<Initials>J</Initials>
<AffiliationInfo><Affiliation>Jilin Academy of Agricultural Sciences, Changchun, 130033, China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Zhong</LastName>
<ForeName>Xiaofang</ForeName>
<Initials>X</Initials>
<AffiliationInfo><Affiliation>Jilin Academy of Agricultural Sciences, Changchun, 130033, China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Kim</LastName>
<ForeName>Kyung Seok</ForeName>
<Initials>KS</Initials>
<AffiliationInfo><Affiliation>Iowa State University, Ames, IA, 50011, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Yang</LastName>
<ForeName>Jing</ForeName>
<Initials>J</Initials>
<AffiliationInfo><Affiliation>Jilin Academy of Agricultural Sciences, Changchun, 130033, China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Xing</LastName>
<ForeName>Guojie</ForeName>
<Initials>G</Initials>
<AffiliationInfo><Affiliation>Jilin Academy of Agricultural Sciences, Changchun, 130033, China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Li</LastName>
<ForeName>Xiaoyu</ForeName>
<Initials>X</Initials>
<AffiliationInfo><Affiliation>Jilin Academy of Agricultural Sciences, Changchun, 130033, China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Jiang</LastName>
<ForeName>Zhaoyuan</ForeName>
<Initials>Z</Initials>
<AffiliationInfo><Affiliation>Jilin Academy of Agricultural Sciences, Changchun, 130033, China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Li</LastName>
<ForeName>Qiyun</ForeName>
<Initials>Q</Initials>
<AffiliationInfo><Affiliation>Jilin Academy of Agricultural Sciences, Changchun, 130033, China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Dong</LastName>
<ForeName>Yingshan</ForeName>
<Initials>Y</Initials>
<AffiliationInfo><Affiliation>Jilin Academy of Agricultural Sciences, Changchun, 130033, China. ysdong@cjaas.com.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Pan</LastName>
<ForeName>Hongyu</ForeName>
<Initials>H</Initials>
<AffiliationInfo><Affiliation>College of Plant Science, Jilin University, Changchun, 130062, China. panhongyu@jlu.edu.cn.</Affiliation>
</AffiliationInfo>
</Author>
</AuthorList>
<Language>eng</Language>
<PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType>
<PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType>
</PublicationTypeList>
<ArticleDate DateType="Electronic"><Year>2018</Year>
<Month>05</Month>
<Day>04</Day>
</ArticleDate>
</Article>
<MedlineJournalInfo><Country>Netherlands</Country>
<MedlineTA>Transgenic Res</MedlineTA>
<NlmUniqueID>9209120</NlmUniqueID>
<ISSNLinking>0962-8819</ISSNLinking>
</MedlineJournalInfo>
<ChemicalList><Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D001425">Bacterial Outer Membrane Proteins</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>151217-41-3</RegistryNumber>
<NameOfSubstance UI="C087295">HrpZ protein, Pseudomonas syringae</NameOfSubstance>
</Chemical>
</ChemicalList>
<CitationSubset>IM</CitationSubset>
<MeshHeadingList><MeshHeading><DescriptorName UI="D001425" MajorTopicYN="N">Bacterial Outer Membrane Proteins</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D060467" MajorTopicYN="N">Disease Resistance</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D018506" MajorTopicYN="N">Gene Expression Regulation, Plant</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D010838" MajorTopicYN="N">Phytophthora</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000472" MajorTopicYN="Y">pathogenicity</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D010935" MajorTopicYN="N">Plant Diseases</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000469" MajorTopicYN="N">parasitology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D018547" MajorTopicYN="N">Plant Stems</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName>
<QualifierName UI="Q000469" MajorTopicYN="N">parasitology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D030821" MajorTopicYN="N">Plants, Genetically Modified</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName>
<QualifierName UI="Q000469" MajorTopicYN="N">parasitology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D044224" MajorTopicYN="N">Pseudomonas syringae</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D015398" MajorTopicYN="N">Signal Transduction</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D013025" MajorTopicYN="N">Soybeans</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName>
<QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName>
<QualifierName UI="Q000469" MajorTopicYN="N">parasitology</QualifierName>
</MeshHeading>
</MeshHeadingList>
<KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="Y">Disease resistance</Keyword>
<Keyword MajorTopicYN="Y">Harpin protein</Keyword>
<Keyword MajorTopicYN="Y">Phytophthora sojae</Keyword>
<Keyword MajorTopicYN="Y">Transgenic plant</Keyword>
</KeywordList>
</MedlineCitation>
<PubmedData><History><PubMedPubDate PubStatus="received"><Year>2017</Year>
<Month>03</Month>
<Day>27</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="accepted"><Year>2018</Year>
<Month>04</Month>
<Day>04</Day>
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<Month>5</Month>
<Day>8</Day>
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<affiliations><list><country><li>République populaire de Chine</li>
<li>États-Unis</li>
</country>
<region><li>Iowa</li>
<li>Jilin</li>
</region>
<settlement><li>Ames (Iowa)</li>
<li>Changchun</li>
</settlement>
<orgName><li>Université d'État de l'Iowa</li>
<li>Université de Jilin</li>
</orgName>
</list>
<tree><country name="République populaire de Chine"><region name="Jilin"><name sortKey="Du, Qian" sort="Du, Qian" uniqKey="Du Q" first="Qian" last="Du">Qian Du</name>
</region>
<name sortKey="Dong, Yingshan" sort="Dong, Yingshan" uniqKey="Dong Y" first="Yingshan" last="Dong">Yingshan Dong</name>
<name sortKey="Du, Qian" sort="Du, Qian" uniqKey="Du Q" first="Qian" last="Du">Qian Du</name>
<name sortKey="Jiang, Zhaoyuan" sort="Jiang, Zhaoyuan" uniqKey="Jiang Z" first="Zhaoyuan" last="Jiang">Zhaoyuan Jiang</name>
<name sortKey="Li, Qiyun" sort="Li, Qiyun" uniqKey="Li Q" first="Qiyun" last="Li">Qiyun Li</name>
<name sortKey="Li, Xiaoyu" sort="Li, Xiaoyu" uniqKey="Li X" first="Xiaoyu" last="Li">Xiaoyu Li</name>
<name sortKey="Pan, Hongyu" sort="Pan, Hongyu" uniqKey="Pan H" first="Hongyu" last="Pan">Hongyu Pan</name>
<name sortKey="Xing, Guojie" sort="Xing, Guojie" uniqKey="Xing G" first="Guojie" last="Xing">Guojie Xing</name>
<name sortKey="Yang, Jing" sort="Yang, Jing" uniqKey="Yang J" first="Jing" last="Yang">Jing Yang</name>
<name sortKey="Yang, Xiangdong" sort="Yang, Xiangdong" uniqKey="Yang X" first="Xiangdong" last="Yang">Xiangdong Yang</name>
<name sortKey="Zhang, Jinhua" sort="Zhang, Jinhua" uniqKey="Zhang J" first="Jinhua" last="Zhang">Jinhua Zhang</name>
<name sortKey="Zhong, Xiaofang" sort="Zhong, Xiaofang" uniqKey="Zhong X" first="Xiaofang" last="Zhong">Xiaofang Zhong</name>
</country>
<country name="États-Unis"><region name="Iowa"><name sortKey="Kim, Kyung Seok" sort="Kim, Kyung Seok" uniqKey="Kim K" first="Kyung Seok" last="Kim">Kyung Seok Kim</name>
</region>
</country>
</tree>
</affiliations>
</record>

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Over-expression of the Pseudomonas syringae harpin-encoding gene hrpZm confers enhanced tolerance to Phytophthora root and stem rot in transgenic soybean.

Over-expression of the Pseudomonas syringae harpin-encoding gene hrpZm confers enhanced tolerance to Phytophthora root and stem rot in transgenic soybean.

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